We also would want to thank the Takeda Science Foundation to the monetary guidance of the analyze. We wish to thank many of the customers with the laboratory for valuable dialogue. We would like to thank Jun Horiuchi for significant looking through of your manuscript.
14) The Discussion is actually a repeat of the Results part with citation of figures and details all over again. It may be shortened and include things like discussion of former data and The brand new observations.
Potential roles of Cdc7 in checkpoint activation were being noted in yeast (Takeda et al., 2001), but outcomes of Cdc7 downregulation on origin firing have manufactured it tough to attract a definitive conclusion about the purpose of Cdc7 in checkpoint activation. Generation of bypass mutations of cdc7
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We agree Along with the remark and redone the experiment. The new facts in Figure 2-figure supplement one present apparent maximize of Chk1 binding to your peptide on phosphorylation by Cdc7.
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. Raw imagining datasets are available upon request. Resource details for western blots and flow cytometry gating strategies are supplied with this particular paper.
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Right after washing, samples were analyzed by western blotting employing anti-Chk1 antibody. Purified Cdc7 protein and ATP were being also extra along with purified Chk1 protein in the event the template peptides needed to be phosphorylated.
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